We have provided many of our most commonly used protocols as a resource to those who wish to confirm our results or expand on our published work. We make every effort to post the most up-to-date information possible. If you are working on a particular technique and have any questions or concerns, please contact us and we will make every effort to put you in touch with a team member who may be working with the method currently.
Know that an informed decision on your part to modify a protocol from what is our current lab practice means you will face certain tragic scientific defeat, or at least a lack of assistance from us and a snarky response to inquires, as we aren't in the business of reinventing wheels. We leave that to Firestone, and they seem to be having enough problems.
Now enjoy a protocol or two thousand on us.
All new members of the team are expected to ensure they are compliant with VUMC policies for safety, animal handling, human specimen work and that they have a full understanding of of lab maintenance and business policies. For more information, be sure you check the McLaughlin Lab Guidelines.
Neuronal dissection videos are available from companies like Stem Cell Technology
From this demo, you can get a sense of the prep though.