Transfusion Medicine FAQs




  • What is the mechanism of action for Uvadex, the medication used in photopheresis

Methoxsalen (8-MOP) is a medication injected into the collection bag during the photopheresis procedure. This medication creates covalent bonds to pyrimidine bases in DNA, inhibiting the synthesis of DNA, and suppressing cell division. The augmented sunburn reaction involves excitation of the methoxsalen molecule by radiation in the long-wave ultraviolet light (UVA), resulting in transference of energy to the methoxsalen molecule producing an excited state. This medication is most frequently isolated from the fig plant.

  • ECP Related questions, contact Lung Transplant Nurse Practitioner:

Laura Roberts: pager, 835-7864 OR Jean Barnes: pager, 835-9219 OR Jennifer Crichton, pager 865-8900

Blood Donor

Blood Volume


  • How much Factor V exists in Fresh Frozen Plasma (FFP)?
    Factor V levels in FFP can vary slightly with ABO group. On average the Factor V level in FFP is 100 IU/dL. The half-life of Factor V is ~12 hours.



Per FDA standards a single apheresis platelet product must have less than 2 mL of RBC in an apheresis platelet product. If the product has more than 2 mL of RBCs then the product would require crossmatching. VUMC uses apheresis platelets that contain less than 2 mL of RBCs.

  • Suspected Neonatal Alloimmune Thrombocytopenia:

BCW_NAIT form.pdf NAIT flow sheet BG_PP.pdf

  • Suspected platelet refractoriness:

Flowsheet ARC form

DCI platelet refractory order form

Product Modification


Shelf LIfe of Red Blood Cells based on different anticoagulant/preservative solutions

Anticoagulant/Preservative Solution:

  • ACD/CPD/CP2D: 21 days
  • CPDA-1: 35 days
  • AS: 42 days

Transfusion Complication


Laboratory FAQs

  Bloody Truth


The product is PLASMA-LYTE A (BAXTER) Injection pH 7.4 (Multiple Electrolytes Injection, Type 1, USP).  It is compatible with blood and blood components and can be administered prior to or following the infusion through the same tubing set, and it can also be infused through the same line during blood transfusion.

  • How long does it take to perform an antibody screen?

    The minimum time needed for an antibody screen to be completed is 60 minutes. For complex immunohematologic evaluations, additional time may be required.
  • Lab Medicine Video
  • My patient has a positive antibody screen. Can I give O Negative blood if necessary?
  • If a patient has a positive antibody screen, it means they have developed an antibody to an antigen other than the ABO/Rh antigens. There are thousands of red cells antigens, and the only way to be certain the red blood cells (RBCs) will be compatible is to complete the antibody work up and crossmatch antigen negative blood.

  • Transfusion Administration Record TAR Education Form 

  Transfusion Complication

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